Genetic fingerprinting of microbial communities – present status and future perspectives
نویسنده
چکیده
The use of ribosomal RNA (rRNA) as molecular marker to identify microbial populations is now routinely used in microbial ecology. One of the strategies herein is cloning of PCR products obtained from environmental DNA to explore the diversity of microbial communities without the need of cultivation. Although successful, this approach is for practical reasons not suited to study the complex dynamics which microbial communities can undergo by diel or seasonal fluctuations or after environmental perturbations. For this purpose, genetic fingerprinting techniques are better tools. Here I present the current status of the application of genetic fingerprinting techniques in microbial ecology and give an outlook into the future.
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